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Results of one representative out of three to five independent experiments are shown.Full figure and legend (65K)To show whether NO may directly affect the DNA binding activity of the AR, nuclear extracts of 22Rv1 cells treated with 10 nM R1881 for 30 h were isolated and incubated with increasing concentrations of the short living spontaneous NO donor (Z) 1 (N,N diethylamino)diazen 1 ium 1,2 diolate (DEA/NO) (half life at room temperature (RT): about 16 min) (Keefer et al., 1996). DEA/NO inhibited the AR DNA binding activity in a concentration dependent manner (Figure 5c). More than 90% inhibition was found using 500 M DEA/NO, whereas 500 M of the denitrosated control compound DEA/NO NO showed almost no effect. lida daidaihua what to eat while taking it When I entered the facility that day, I was first greeted by a man on crutches who told me he had just broken his ankle in an outdoor climbing fall. He seemed pretty intense. There were several men climbing near the front without shirts on, their bodies seemingly made of nothing but muscle and sinew.
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