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For the functional analysis of CNEs in developing chick cornea, stage HH25 embryos were electroporated with the respective GFP reporter constructs for CNE 2 to CNE 5. Platinum electrodes were positioned with the cathode placed adjacent to the cornea of the right eye and the anode behind the head. A Hamilton needle with a blunt syringe was used to dispense 0.5 of a DNA glycerol mix (containing 17 GFP reporter construct, 17 RFP expression construct, 65 glycerol, 1 fast green) onto the cornea of the right eye of the embryo. Garcinia cambogia weight loss Facebook has the news feed. Google is reportedly working on the activity stream. Facebook has chat. Zoe, my Jack Russell terrier, never had so many walks in her life. She seemed as excited by my new toy as I was, especially when it vibrated and flashed in celebration when I reached my daily goal.My Fitbit honeymoon came to a crashing end after about eight weeks, when it became clear that my regular dog walks were barely getting me to the 10,000 step mark as my Fitbit “told”me. If Zoe and I missed one walk, I missed my goal and that left me unhappy.
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