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The 1,536 well format also permits a quantitative HTS approach that uses an automated, robotic system to screen the large collection of compounds in a 4 point dose response23. This reduces the number of false positive hits and enables the identification of compounds that are less potent, but are still potentially relevant24.Figure 2: The primary 3D organotypic HTS in 384 well and 1,536 well formats.(a) HTS assay (384 well). The Prestwick Chemical Library of compounds at a final concentration of 10M (n=2 experiments, 1,140 compounds) was added to the 384 well 3D HTS assay. meizitang strong version does it work Results of one representative out of three to five independent experiments are shown.Full figure and legend (65K)To show whether NO may directly affect the DNA binding activity of the AR, nuclear extracts of 22Rv1 cells treated with 10 nM R1881 for 30 h were isolated and incubated with increasing concentrations of the short living spontaneous NO donor (Z) 1 (N,N diethylamino)diazen 1 ium 1,2 diolate (DEA/NO) (half life at room temperature (RT): about 16 min) (Keefer et al., 1996). DEA/NO inhibited the AR DNA binding activity in a concentration dependent manner (Figure 5c). More than 90% inhibition was found using 500 M DEA/NO, whereas 500 M of the denitrosated control compound DEA/NO NO showed almost no effect.