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Microporous materials have been produced by gradual precipitation from solutions of poly(var epsilon caprolactone) (PCL) in acetone induced by solvent extraction across a semi permeable PCL membrane which is formed in situ at the polymer solution/non solvent interface. Microparticulates of hydroxyapatite and inulin polysaccharide, respectively, were incorporated in precipitation cast PCL matrices to illustrate potential applications in hard tissue repair and macromolecular drug release. Microporous PCL and HA filled PCL materials were found to provide a favourable surface for attachment and growth of primary human osteoblasts in cell culture. The in vitro degradation characteristics of microporous PCL and inulin/PCL materials in PBS at 37C were monitored over 45 months. Microporous PCL demonstrated zero weight loss, minor changes in molecular weight characteristics and a fairly constant indentation resistance of around 1 MN/m2. Inulin loaded PCL materials exhibited a total weight loss of approximately 17% after 12 months in PBS. The indentation resistance decreased by 50% from an initial value of 28 MN/m2 in the first 2 months and then remained stable. Precipitation cast materials based on PCL are expected to be useful for formulating long term, controlled release devices for bioactive molecules such as growth factors and hormones and extended residence supports for cell growth and tissue development. Mum raped by web beast in front of son My doctor asks me if something hurts and I dont know. I forget what it feels like for things to not hurt.
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