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The design features included (16) (i) consecutive sampling of female patients; (ii) development and use of a standard questionnaire with operational definitions of abuse and validation by interview; (iii) administration of psychosocial assessment instruments to further characterize the study group; (iv) validation of medical diagnoses by the patients physicians; (v) GI symptom assessment using diary cards and motility testing; and (vi) health status and utilization outcome assessment every 3 months using mailings over 1 year. However, because of the rigor of the design and the nature of assessment, only 33 of the subjects completed the study. For the most part, those screened for the study were similar to those completing the study, except that the latter group had a higher frequency of sexual abuse (49.4 vs. The Process of Patenting an Idea Though the spheroids can recapitulate some aspects of cancer cell growth in 3D, their variable growth patterns make them unsuitable for HTS8,9. Spheroids also cannot replicate bidirectional interactions between tumour cells, stromal cells and the ECM10,11,12,13. Interestingly, tumour cells grown in a multi cell type 3D organotypic model show significant differences in drug sensitivity when compared with cancer cells grown in single cell type 3D spheroids or as a monolayer11.
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