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(d) Flow chart for 3D HTS screening platform including the primary, confirmatory, counter and secondary biological assays (in vitro and in vivo). The criteria for compound selection and the number of compounds at each step are listed.The HTS assay was adapted into a 384 well format through optimization of several parameters, including the incubation time (16h), number of cancer cells (12,000), mesothelial cells (4,000) and fibroblasts (400) (Supplementary Fig. 2a c). zi xiu bee pollen chinese diet pills His point is that there’s more to social media than clever campaigns and rudimentary conversations. Talking isn’t the only thing that makes social media social. Just like adding Facebook, Twitter and other sharing buttons will not magically transform static content into shareable experiences.
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