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The principal sites of OvCa metastasis are the omentum, a large fat pad (20 20 3cm) that covers the bowel in the peritoneal cavity, and the peritoneum, the serous membrane that lines the peritoneal cavity17.In this study, our goal was to recapitulate the structural organization and function of the superficial tissue layers encountered by OvCa cells during metastasis in an assay amenable to drug screening using a HTS platform. Our hypothesis was that by adapting a 3D system that more faithfully represents the histologic and biologic complexity of the most common sites of OvCa metastasis for HTS, we could more reliably identify those compounds that effectively inhibit metastasis in vivo. We developed a heterotypic organotypic culture assay that mimics the surface histology of the omentum and peritoneum using primary cells, and used it to evaluate the effects of small molecule compounds on the adhesion, migration and invasion of OvCa cells. meizitang amazon jp Be realistic and get perspective. The summer is only three months at most. That leaves nine other months, so of course you couldn’t get all the projects done or do all the vacationing you ever wanted in such a short time. And as most people know, casinos often have great bars and restaurants themselves. Whether you end up spending the whole night at a casino is up to you. But casinos will most likely prove a lot of fun for you and your friends on a stag night.
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