Such accidents are a common household occurrence and cost a lot in the form of increased insurance premiums. All you need to do is put up some solar garden lamps, light up your house, and forget about any bills. It will also reduce your insurance premium.. , activity health New qPCR primer sets targeting dsrA and genes were designed according to the manufacturer specifications (Applied Biosystems, Carlsbad, CA, USA) and had amplicon sizes ranging from100 to 200 qPCR primers targeting all protozoan genes found in the groundwater (qbetGen_260F were designed from sequences found in our clone libraries and from representative protozoan sequences obtained from the GenBank database.Primers for qPCR were also designed to target specifically Breviata and Hexamita genes, and Desulfosporosinus dsrA genes in the groundwater (Supplementary Table S2). The Breviata primer pair (qbetBrev_521F was designed from a clone (clone RB) that had 86 nucleotide identity to from Breviata anathema and accounted for 65 of the clone library assembled from groundwater collected on day 14 during the 2010 field experiment. The Geobacter gltA primer pair (CS375F was previously designed from a Geobacter sequence most similar to Geobacter sp.
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