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The 1,536 well format also permits a quantitative HTS approach that uses an automated, robotic system to screen the large collection of compounds in a 4 point dose response23. This reduces the number of false positive hits and enables the identification of compounds that are less potent, but are still potentially relevant24.Figure 2: The primary 3D organotypic HTS in 384 well and 1,536 well formats.(a) HTS assay (384 well). The Prestwick Chemical Library of compounds at a final concentration of 10M (n=2 experiments, 1,140 compounds) was added to the 384 well 3D HTS assay. cabalonga blood pressure Top of pageAbstractMaterials and methods Results Discussion References Acknowledgements Figures and TablesCancer invasion is regulated by cell surface proteinases and adhesion molecules. Interaction between specific cell surface molecules such as urokinase plasminogen activator receptor (uPAR) and integrins is crucial for tumour invasion and metastasis. In this study, we examined whether uPAR and integrin form a functional complex to mediate signalling required for tumour invasion.
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