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The patterns of cell surface expression of uPAR and integrin subunit on HCT116 clones were assessed by flow cytometry using anti uPAR and anti integrin antibody. The expression of cell surface uPAR in WT and mock transfected cells was two fold higher than in A clone, but there was no change in the expression of integrin (Figure 1B). There was no difference in the proliferative response of mock and A cells (Table 1). ) how to use dai dai hua jiao nang Our results demonstrate that an in vitro 3D model based on primary cells can be used effectively in HTS and could become an important tool for improving drug screening efficacy.ResultsEstablishment of an organotypic HTS assayAll organs in the peritoneal cavity are covered by a single layer of mesothelial cells that rest on a basement membrane of ECM interspersed with fibroblasts (Fig. 1a)20. A heterotypic, 3D organotypic culture assay mimicking the surface histology of the omentum and peritoneum was assembled with primary human mesothelial cells and fibroblasts mixed with ECM (fibronectin and collagen type I).
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