Before R spectral analysis was performed, the stored R interval data were displayed and aligned sequentially to obtain equally spaced samples with an effective sampling frequency of 2 Hz,29 and displayed on a computer screen for visual inspection. Then, the direct current component and linear trend were completely eliminated by digital filtering for the band pass between 0.03 and 0.5 Hz. The root mean square value of the R interval was calculated as representing the average amplitude. ) Plant weight loss Incubation with increasing concentrations of S nitrosocysteine, another spontaneous NO donor, as well as experiments using nuclear extracts of LNCaP prostate cancer cells yielded similar results (data not shown). In summary, these results show that NO inhibits the AR DNA binding activity in a concentration dependent manner.Prostate cells which do not express AR are less susceptible to NOEffects of NO on cell proliferation were tested with AR negative DU 145 cells as well as with AR positive LNCaP and 22Rv1 prostate cancer cells (Stone et al., 1978; Horoszewicz et al., 1983; Sramkoski et al., 1999). In contrast to LNCaP, which depend on androgenic stimuli for growth, proliferation of 22Rv1 cells is only weakly stimulated by androgens, although they are able to proliferate in the absence of androgens (Sramkoski et al., 1999; Attardi et al., 2004).
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