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The degrees of OTU sharing and unsharing were determined, respectively, for both healthy and caries active populations. The unshared OTU curves suggested that each additional sample from either group contributed more diversity to the pool (Figure 1c). The shared OTU curves revealed a gradual decrease of OTU sharing with the individual addition of hosts (Figure 1c). Ann Powers Top Albums And Songs Of 2012 Put together a profile. Include videos of speeches you’ve given, PowerPoint presentations, a video r even. Highlight any volunteer work you’re doing to fill the gaps in your r and frame it in business terms. More recently, Plaque Hypothesis (Marsh, 1994, 2003, 2006a) emerged and proposed that caries resulted from a populational balance shift of the resident microbiota, driven by environmental changes in oral conditions. Culture independent methods for bacterial identification and enumeration such as array based DNA hybridization (Corby et al., 2005; Aas et al., 2008; Kanasi et al., 2010) or Sanger sequencing of 16S rRNA gene clones (Aas et al., 2005) have revealed a wide array of bacteria correlated with caries progression.In this study, we report the most in depth, comprehensive and collaborated view to date of the organismal lineage of oral microbiota in both caries active and normal human populations. Our results from 16S rRNA gene amplicon based sequencing were cross validated by whole genome based deep sequencing technologies.