Messenger RNA (mRNA), which carries the genetic code for proteins, is present in bacterial and eukaryotic cells. In eukaryotes, it’s found in the nucleus and cytoplasm. Transfer RNA (tRNA), which helps incorporate amino acids into growing polypeptide chains, is present in bacterial and eukaryotic cells. # Bodybuilder and Her Fired TV Producer Press Lawsuits 5 and 25 of the 18S rRNA and clone libraries (see Supplementary Material, Supplementary Figure S2 and Supplementary Table S1). In all, 100 plasmid inserts from each of these clone libraries were sequenced with the M13F primer at the University of Massachusetts Sequencing Facility.Calculation of diversity indicesThe Shannon and Simpson indices of diversity were used to determine the diversity of taxa present in groundwater collected from the site. The Shannon diversity index (H was calculated as follows (Margalef, 1958; Dunbar et al., 1999):where pi in both of these equations represented the proportion of the ith phylotype.Testing and design of qPCR primersThe following primer sets were used to quantify 16S rRNA and citrate synthase (gltA) gene and mRNA transcript copies found in groundwater collected during both field experiments by quantitative (q)PCR: 16S rRNA was amplified with 338F (Weisburg et al., 1991) and 518R (Muyzer et al., 1993), and gltA was amplified with CS375F and CS598R (Holmes et al., 2005).
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