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Immunohistochemical stainings for iNOS and nitrotyrosine were specific, as evidenced by negative control reactions, in which the respective primary antibodies were omitted (Figures 1c and d). Our results indicate that iNOS expressed in human prostate cancer is fully active and induces nitrosative stress. In prostate cancer cells iNOS (a) and nitrotyrosine (b) were coexpressed as shown by staining of serial prostate cancer sections. fruta planta china tennis Another great way to help the environment is by sharing coupons with your friends and family the electronic way. A lot of sites offer multiple ways to “share” the coupon or discount offer via email, Facebook, MySpace, Twitter and other social networking sites. In the old days, people would cut out coupons and sometimes, even mail them to friends and family through the postal service costing money and wasting paper..