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To analyse whether NO affects the genomic (transcriptional) response to androgen, AR positive human prostate cancer cells (22Rv1) were transiently cotransfected with an androgen responsive probasin promoter driven reporter plasmid (pGL3E Pro) and a pRL tk LUC Renilla vector as an internal control (Tepper et al., 2002; Cronauer et al., 2004). Subsequently, cells were grown for 30 h in the presence of 10 nM of the synthetic androgen methyltrienolone (R1881) together with increasing concentrations of the DETA/NO for 30 h. DETA/NO inhibited transcriptional AR activity in a concentration dependent manner (Figure 2a). . zi xiu tang bee pollen coupon code muscle but one thing’s for sure
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