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Medical officials in Gaza said at least 60 civilians, including a four year old girl and a boy of five killed on Thursday, were among the 82 Palestinians who have died since the offensive began on Tuesday. President Barack Obama told Netanyahu by telephone on Thursday that the United States was willing to help negotiate a ceasefire, the White House said.. the bee pollen girls llc Therapeutic Silencing of Bcl 2 by Systemically Administered siRNA Nanotherapeutics Inhibits Tumor Growth by Autophagy and Apoptosis and Enhances the Efficacy of Chemotherapy in Orthotopic Xenograft Models of ER ( and ER (+) Breast CancerIbrahim Tekedereli, S Neslihan Alpay, Ugur Akar, Erkan Yuca, Cristian Ayugo Rodriguez, He Dong Han, Anil K Sood, Gabriel Lopez Berestein and Bulent OzpolatTherapeutic silencing of Bcl 2 by NL Bcl 2 siRNA inhibits in vivo tumor growth of ER( MDA MB 231 xeonografts in nude mice. (a) Mice were orthotopically injected into mammary fat pat with luciferase expressing MDA MB 231 cells. Mice bearing tumors with equal size were randomly assigned into control and treatment groups (n = 5 mice per group). (b) Therapeutic silencing of Bcl 2 by NL Bcl 2 siRNA enhances the antitumor efficacy of doxorubicin. (c) Mice that were treated with NL Bcl 2 siRNA had significantly reduced tumor weight compared with the control group that was treated with NL Control siRNA. Enhanced the antitumor efficacy of doxorubicin was observed when combined with NL Bcl 2 siRNA. The tumor weights were measured 4 weeks of treatments shown in Figure 3a, b. (d) Bcl 2 protein expression after 4 weeks of treatments in MDA MB 231 tumors. After sacrificing mice, tumors were removed (48 hours after the last treatment) and tumor lysates were analyzed for Bcl 2 expression by western blot. (e) NL Bcl 2 siRNA treatment was well tolerated and did not cause weight lose in mice, compared with those that received NL control siRNA. Mice that received doxorubicin had slight reduced weight loss compared with those that did not receive chemotherapy. (f) In vitro silencing of Bcl 2 by siRNA treatment increases the antiproliferative effects of paclitaxel and inhibit colony formation of MDA MB 231 cells.
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